Serial Dilution in 96 & 384 well Microplates

Dilugent Shaker

Serial Dilution | Definition, Purpose & Calculation

Jo | Assistant Professor | PhD Scholar on Instagram: "Calculation in comments. Have you tried this method? 🧪 “Step 1: Do serial dilutions” 📍 “Step 2: Divide plate into sections” 💧 “Step 3: Add 10 µL drops per dilution” 🕒 “Step 4: Let dry → Incubate” 🔢 “Step 5: Count colonies → Calculate CFU/mL” 📌 “Saves media, plates, and TIME!” Tips for accuracy: ✅ Use at least 3 drops per dilution for average. ✅ Choose drops where colonies are countable (ideally 3–30 colonies). ✅ Avoid drops where coloni