High-salt wash buffer: 500 mM NaCl, 0.1% SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl (pH 8) LiCl wash buffer: 0.25 M LiCl, 1% NP-40 (or IGEPAL CA-360), 1% DOC, 1 mM EDTA, 10 mM Tris-HCl (pH 8) Low ...
One evening in the early 1970s, Michael Pachovas and a few friends wheeled themselves to a curb in Berkeley, Calif., poured cement into the form of a crude ramp, and rolled off into the night. 1 For ...
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